IL-10 treatment of macrophages bolsters intracellular survival of Mycobacterium leprae.
نویسندگان
چکیده
In these studies, metabolically active Mycobacterium leprae were maintained for as long as 8 weeks in monolayer cultures of mouse peritoneal macrophages (MPhi). Supplemental IL-10, but not TGF-beta, bolstered, directly or indirectly, M. leprae metabolism in mouse MPhi. In the cell culture system temperature setting is extremely important and 31 to 33 degrees C incubation temperature was more permissive than 37 degrees C. Acid fast staining and transmission electron microscopy (TEM) of intracellular M. leprae revealed visible elongation of bacilli cultured under the above ideal conditions.
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عنوان ژورنال:
- International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association
دوره 72 1 شماره
صفحات -
تاریخ انتشار 2004